human skin fibroblast  (ATCC)

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Tissue-engineered dermal substitutes constructed by human embryonic stem cell-derived fibroblasts facilitate the repair of skin wounds

doi: 10.3389/fbioe.2026.1777175

Figure Lengend Snippet: Analysis of MSCs Characteristics. (A) Strategy for inducing hESCs differentiation into fibroblasts and constructing tissue-engineered dermal substitutes. (B) The spindle-like morphology of hESC-MSCs (a) and hMSCs (b) . Scale bar = 100 μm. (C) Flow cytometric analysis of cell surface antigen expression on hESC-MSCs (a) and hMSCs (b) . (D) Quantitative analysis of MSC cell surface markers (data represent mean ± SD, n = 3). ** p < 0.01.

Article Snippet: Human skin fibroblasts (HSF, ATCC PCS-201–012®TM) were used as a positive control.

Techniques: Expressing

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Tissue-engineered dermal substitutes constructed by human embryonic stem cell-derived fibroblasts facilitate the repair of skin wounds

doi: 10.3389/fbioe.2026.1777175

Figure Lengend Snippet: Fibroblastic differentiation of hESC-MSCs. (A) Cellular morphology of hESC-Fbs and HSF. Scale bar = 100 μm. (B) Immunofluorescence analysis of VIM and CK5 expression in hESC-Fbs and HSF. VIM and CK5 were shown with green, nuclei were counterstained with blue. Scale bar = 100 μm. (C) qPCR assay of fibroblast relevant markers during fibroblastic differentiation of hESC-MSCs (data represent mean ± SD, n = 3). * p < 0.05 and ** p < 0.01 vs. hESC-MSCs. (D) ELISA assay of fibroblast relevant markers during fibroblastic differentiation of hESC-MSCs (data represent mean ± SD, n = 3). ** p < 0.01 vs. hESC-MSCs.

Article Snippet: Human skin fibroblasts (HSF, ATCC PCS-201–012®TM) were used as a positive control.

Techniques: Immunofluorescence, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Sensors (Basel, Switzerland)

Article Title: A Heptamethine Cyanine-Based Near-Infrared Optical Sensor for Copper(II) Detection in Aqueous Solutions and Living Cells

doi: 10.3390/s26010130

Figure Lengend Snippet: Confocal microscopy images of WS1 fibroblast cells. Bright-field image ( A ) and confocal fluorescence image ( B ) of WS1 cells incubated with IRPhen (10 µM) for 30 min. Bright-field image ( C ) and confocal fluorescence image ( D ) of cells pretreated with Cu 2+ (10 µM) for 8 h, followed by incubation with IRPhen (10 µM) for 30 min; ( E ) Quantitative bar graph showing the corresponding fluorescence intensities of panels ( B , D ). Excitation was at 633 nm, and emission was collected from 650–850 nm. Scale bar, 10 µm.

Article Snippet: Human skin primary fibroblast cells (WS1) purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) were used in this study.

Techniques: Confocal Microscopy, Fluorescence, Incubation

Journal: Aging Cell

Article Title: Single‐Cell Fluorescence Imaging Reveals Heterogeneity in Senescence Biomarkers and Identifies Rapamycin‐Responsive Sub‐Populations

doi: 10.1111/acel.70209

Figure Lengend Snippet: Assessment of SA‐βgal and cell proliferation in an MMC‐induced senescence model. (A) Representative images of SA‐βgal staining and EDU incorporation in HDFs treated with vehicle and 200 nM MMC. Images obtained with an IN‐Cell analyser 2200 (SA‐βgal) and Opera Phenix plus (EDU). Images show nuclei stained with Hoechst (blue), SA‐βgal (red)/EDU (Green) and merged image (scale bar = 100 μm). (B) Average fluorescence intensities of SA‐βgal in HDFs treated with vehicle (0.1% DMSO) or different concentrations (50–600 nM) of MMC; Error bars represent mean ± standard deviation from three independent biological replicates. * p < 0.05 (Simple one‐way ANOVA compared with the (0) control group). (C) Percentage of cells positive for EDU incorporation in HDFs treated with MMC; **** p < 0.0001, (Student unpaired t ‐test compared with 0 group). (D) Time course effect of cellular proliferation on HDFs with MMC. (E) Single cell fluorescence intensities of SA‐βgal in HDFs treated with vehicle (0.1% DMSO) or different concentrations (50–600 nM) of MMC. (F) Radar chart of different SA‐βgal fluorescence intensities in HDFs treated with vehicle or different concentrations of MMC. (G) Sub‐population analysis of SA‐βgal fluorescence intensities in HDFs treated with vehicle or 200 nM MMC. (H) Percentage of cells with SA‐βgal intensity of greater than that of the threshold set in the control cells from the respective histograms. Error bars represent mean ± standard deviation from three independent biological replicates. **** p < 0.0001 (Simple one‐way ANOVA compared with the (0) control group).

Article Snippet: Primary human skin fibroblasts (HDFs) (106‐05 N, Sigma‐Aldrich, MO, USA, for Figures and ; or PCS‐201‐010, ATCC, for the remaining figures) were cultured in Dulbecco's Modified Eagle Medium (DMEM, D5523, Sigma‐Aldrich) supplemented with 10% foetal bovine serum (FBS).

Techniques: Staining, Fluorescence, Standard Deviation, Control

Journal: Aging Cell

Article Title: Single‐Cell Fluorescence Imaging Reveals Heterogeneity in Senescence Biomarkers and Identifies Rapamycin‐Responsive Sub‐Populations

doi: 10.1111/acel.70209

Figure Lengend Snippet: Assessment of nuclear area, cell area and P21 expression in an MMC‐induced senescence model in HDFs. Representative images were taken using Opera Phoenix plus at 20× magnification. (A) Representative image of Hoechst‐stained nuclei in HDFs treated with vehicle and 200 nM MMC treated HDFs. Average value of nuclei area (B), cell area (F), P21 (L) in vehicle and MMC treated HDFs; Error bars represent mean ± standard deviation from three independent biological replicates. For Nuclei and cell area (ns, not significant, * p < 0.05 ** p < 0.01) (one‐way ANOVA compared with the (0) control group). For P21 (unpaired t ‐test compared to the control (0) group **** p < 0.0001). Single‐cell data for nuclear area (C) and cell area (G) in HDFs treated with MMC. Individual cell‐derived histogram data categorised into various bin centres for nuclear area (D), cell area (H) and P21 (M) in vehicle‐ and MMC‐treated HDFs. (J) Representative image and quantitation of western blot showing the expression of P21 and β‐Actin, along with the relative quantitiation of expression of P21/β‐Actin expression in MMC‐treated HDFs; * p < 0.05, (Student's unpaired t ‐test compared with 0 control group). (K) Representative images of P21 expression in the nuclei of HDFs treated with vehicle and 200 nM MMC, with nuclei labelled with Hoechst (blue), P21 (red) and merged image (scale bar: 10 μm). Percentage of cells with nuclear area (E), cell area (I) and P21 expression (N) exceeding the threshold set in the control cells, derived from the respective heatmaps in vehicle‐ and MMC‐treated HDFs. Error bars represent the mean ± standard deviation from three independent biological replicates. For nuclear area and cell area (**** p < 0.0001, one‐way ANOVA compared to the control group); For p21 (**** p < 0.0001, unpaired t ‐test compared to the control group).

Article Snippet: Primary human skin fibroblasts (HDFs) (106‐05 N, Sigma‐Aldrich, MO, USA, for Figures and ; or PCS‐201‐010, ATCC, for the remaining figures) were cultured in Dulbecco's Modified Eagle Medium (DMEM, D5523, Sigma‐Aldrich) supplemented with 10% foetal bovine serum (FBS).

Techniques: Expressing, Staining, Standard Deviation, Control, Derivative Assay, Quantitation Assay, Western Blot

Journal: Aging Cell

Article Title: Single‐Cell Fluorescence Imaging Reveals Heterogeneity in Senescence Biomarkers and Identifies Rapamycin‐Responsive Sub‐Populations

doi: 10.1111/acel.70209

Figure Lengend Snippet: Assessment of senescence biomarkers in MMC‐induced senescent HDFs with synchronised cell cycles. Average total fluorescence intensities of SA‐βgal (A), nuclear area (B), cell area (C), nuclear p21 (D) and nuclear p16 (E) in HDFs with synchronised cell cycle treated with vehicle (0.1% DMSO) or different concentrations (50–400 nM) of MMC; Error bars represent mean ± standard deviation from three independent biological replicates. Ns: Not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 (One‐way ANOVA with Dunnet's post hoc analysis; Mean of all group compared with each other). Single cell fluorescence intensities of SA‐βgal (F), nuclear p21 (I) and nuclear p16 (J) in HDFs treated with vehicle (0.1% DMSO) or different concentrations (50–400 nM) of MMC. Single cell data of nuclear area (G) and cell area (H) in HDFs treated with vehicle (0.1% DMSO) or different concentrations (50–400 nM) of MMC.

Article Snippet: Primary human skin fibroblasts (HDFs) (106‐05 N, Sigma‐Aldrich, MO, USA, for Figures and ; or PCS‐201‐010, ATCC, for the remaining figures) were cultured in Dulbecco's Modified Eagle Medium (DMEM, D5523, Sigma‐Aldrich) supplemented with 10% foetal bovine serum (FBS).

Techniques: Fluorescence, Standard Deviation

Journal: Aging Cell

Article Title: Single‐Cell Fluorescence Imaging Reveals Heterogeneity in Senescence Biomarkers and Identifies Rapamycin‐Responsive Sub‐Populations

doi: 10.1111/acel.70209

Figure Lengend Snippet: Correlation analysis of senescence‐associated biomarkers and the impact of cellular heterogeneity on the senescence‐associated secretory phenotype (SASP) in MMC‐induced senescence. Representative correlation plot of SA‐βgal total fluorescence intensity versus nuclear area (A) and ell area (B) for the MMC 200 nM group. Spearman correlation coefficient values for SA‐βgal total fluorescence intensity versus nuclear area (C) and cell area (D). Data represent mean ± standard deviation from three independent biological replicates; ns, not significant ( p > 0.05, ordinary one‐way ANOVA with Dunnett's post hoc test compared to the control group). (E) Representative images of IL‐6 expression in HDFs treated with vehicle and 200 nM MMC with nuclei labelled with Hoechst (blue) and IL‐6 labelled (yellow); merged image (Scale bar: 10 μm). Images were captured using the Opera Phoenix plus at 20× magnification. (F) Average IL‐6 expression in HDFs treated with vehicle or 200 nM MMC. Representative correlation graph of nuclear area (100–300 μm 2 ) versus IL‐6 (RFU) (G) and nuclear area (> 300 μm 2 ) versus IL‐6 (RFU) (H). (I) Average Spearman correlation coefficient ( r ) of nuclear area subpopulations versus IL‐6 fluorescence in HDFs treated with 200 nM MMC. Error bars represent the mean ± standard deviation from three independent biological replicates (unpaired t ‐test, * p < 0.05, **** p < 0.001).

Article Snippet: Primary human skin fibroblasts (HDFs) (106‐05 N, Sigma‐Aldrich, MO, USA, for Figures and ; or PCS‐201‐010, ATCC, for the remaining figures) were cultured in Dulbecco's Modified Eagle Medium (DMEM, D5523, Sigma‐Aldrich) supplemented with 10% foetal bovine serum (FBS).

Techniques: Fluorescence, Standard Deviation, Control, Expressing

Journal: Aging Cell

Article Title: Single‐Cell Fluorescence Imaging Reveals Heterogeneity in Senescence Biomarkers and Identifies Rapamycin‐Responsive Sub‐Populations

doi: 10.1111/acel.70209

Figure Lengend Snippet: Assessment of senescence biomarkers in the MMC‐induced senescence model with rapamycin treatment. Average total fluorescence intensity of SA‐βgal (A), nuclear area (B) and P21 (C) in HDFs treated with either MMC or MMC plus rapamycin. Data represent the mean ± SD from n = 3 biological replicates; *** p < 0.001, **** p < 0.0001 (ordinary one‐way ANOVA compared to the MMC 200 nM treated group). Sub‐population analysis of SA‐βgal (D), nuclear area (E) and p21 (F) in HDFs treated with either MMC or MMC plus rapamycin. Percentage of cells having increased expression of SA‐βgal (G), nuclear area (H) and p21 (I) in HDFs treated either with MMC or MMC plus rapamycin using the induction threshold method. Data represents the mean ± SD from n = 3 biological replicates; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 (ordinary one‐way ANOVA compared to the MMC 200 nM treated group).

Article Snippet: Primary human skin fibroblasts (HDFs) (106‐05 N, Sigma‐Aldrich, MO, USA, for Figures and ; or PCS‐201‐010, ATCC, for the remaining figures) were cultured in Dulbecco's Modified Eagle Medium (DMEM, D5523, Sigma‐Aldrich) supplemented with 10% foetal bovine serum (FBS).

Techniques: Fluorescence, Expressing